(IV) Observation of Microorganisms: • Bright field Microscope, Dark Field Microscope, Magnification, Resolution, Numerical aperture, Sample Preparation • Wet Mount, hanging drop technique • Theory of staining: Classification of stains, Stain (Basic and Acidic), Fixative, Mordant, Decoloriser, Accentuator • Principles and methods of staining techniques for following (Monochrome, Negative, Differential (Gram, Acid fast ), Special staining- Endospore, flagella, cell wall, nucleic acid, capsule)
(Unit IV) Observation of Microorganisms: • Bright field Microscope, Dark Field Microscope, Magnification, Resolution, Numerical aperture, Sample Preparation • Wet Mount, hanging drop technique • Theory of staining: Classification of stains, Stain (Basic and Acidic), Fixative, Mordant, Decoloriser, Accentuatorj • Principles and methods of staining techniques for following (Monochrome, Negative, Differential (Gram, Acid fast ), Special staining- Endospore, flagella, cell wall, nucleic acid, capsule) • Bright field Microscope, Dark Field Microscope, Magnification, Resolution, Numerical aperture, Sample Preparation The early pioneers of microscopy opened a window into the invisible world of microorganisms. But microscopy continued to advance in the centuries that followed. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy, which uses an ultravio